Buffer Dilution From Stock
Calculator
Stock volume needed (mL)
10
Results
- Stock volume needed (mL)
- 10
- Diluent volume needed (mL)
- 490
- Dilution factor
- 50
Biology and lab results
| Stock volume needed (mL) | 10 |
| Diluent volume needed (mL) | 490 |
| Dilution factor | 50 |
formula-map diagram
- Stock volume needed (mL)
- 10
- Diluent volume needed (mL)
- 490
- Dilution factor
- 50
Formula breakdown
Formula
C₁V₁ = C₂V₂ → V₁ = (C₂ × V₂) ÷ C₁= 10
Note
Simplified model: these results use standard textbook laboratory relationships and average constants (A260 = 1 for 50 µg/mL dsDNA, 617.96 g/mol per base pair, ~110 Da per amino acid, ideal exponential growth). Real samples vary with purity, contaminants, buffer, temperature and instrument calibration. Always confirm against your own standards and protocol; do not use for diagnostic or safety-critical work.